{"id":4609,"date":"2020-09-23T16:31:40","date_gmt":"2020-09-23T20:31:40","guid":{"rendered":"https:\/\/mrm.research.mcgill.ca\/event\/biochemistry-department-seminar-dr-timothy-j-stasevich\/"},"modified":"2021-01-05T10:33:14","modified_gmt":"2021-01-05T15:33:14","slug":"seminaire-du-departement-de-biochimie-dr-timothy-j-stasevich","status":"publish","type":"scrm_events","link":"https:\/\/mrm.research.mcgill.ca\/fr\/event\/seminaire-du-departement-de-biochimie-dr-timothy-j-stasevich\/","title":{"rendered":"S\u00e9minaire du D\u00e9partement de biochimie : Dr. Timothy J. Stasevich"},"content":{"rendered":"

Lundi 5 Octobre 2020, 16h<\/strong><\/p>\n

\"\"

Dr. Stasevich<\/p><\/div>\n

Timothy J. Stasevich, Ph.D. Professeur agr\u00e9g\u00e9<\/strong>
\nProfesseur, CSU Monfort
\nD\u00e9pertement de biochimie et de biologie mol\u00e9culaire, Universit\u00e9 d\u2019\u00c9tat du Colorado (CSU)
\nFort Collins, CO, 80523 USA
\nSite web: http:\/\/sites.bmb.colostate.edu\/stasevichlab<\/a><\/p>\n

Titre : \u201cReal-time quantification of gene expression with single-molecule precision in living cells<\/em>\u201d<\/p>\n

 <\/p>\n

Ce s\u00e9minaire se tiendra via Zoom.<\/strong><\/p>\n

Num\u00e9ro du meeting Zoom : 943 77216 239<\/strong><\/p>\n

Rejoindre le s\u00e9minaire<\/a>\n

Mon laboratoire cr\u00e9e une technologie pour imager l\u2019expression des g\u00e8nes en temps r\u00e9el et avec une pr\u00e9cision \u00e0 mol\u00e9cule unique dans les cellules vivantes. \u00c0 l\u2019aide d\u2019ARNm \u00e0 r\u00e9p\u00e9tition en tandem et d\u2019\u00e9tiquettes prot\u00e9iques, d\u2019intracorps g\u00e9n\u00e9tiquement cod\u00e9s et de microscopie \u00e0 mol\u00e9cule unique, nous imageons maintenant r\u00e9guli\u00e8rement la dynamique de traduction en cellule r\u00e9elle des ARNms simples. Dans cette pr\u00e9sentation, je vais introduire notre technologie et d\u00e9crire comment elle peut \u00eatre utilis\u00e9e pour amplifier les signaux fluorescents dans les sites de traduction unique. Je vais montrer comment nous quantifions ces signaux pour d\u00e9terminer la taille, la forme, la localisation subcellulaire et les mobilit\u00e9s des sites de traduction, ainsi que leurs densit\u00e9s ribosomiques et leur cin\u00e9tique d\u2019initiation translationnelle et d\u2019\u00e9longation. Je vais ensuite souligner quelques applications r\u00e9centes de notre technologie, y compris l\u2019imagerie de l\u2019arr\u00eat de la traduction pendant le stress cellulaire, le d\u00e9phasage translationnel du VIH-1 , et l\u2019initiation de la traduction non canonique sur les sites d\u2019entr\u00e9e ribosomales interne. Je conclurai en discutant de nouveaux intracorps que mon laboratoire a con\u00e7u qui lient les \u00e9pitopes classiques HA et FLAG dans les cellules vivantes. Comme ces intracorps peuvent \u00eatre cod\u00e9es sur des plasmides, ils peuvent facilement \u00eatre adapt\u00e9s par d\u2019autres laboratoires \u00e0 la traduction d\u2019images dans de multiples couleurs et dans divers syst\u00e8mes vivants.<\/p>\n

Timothy J. Stasevich est Professeur agr\u00e9g\u00e9 au D\u00e9partement de biochimie et de biologie mol\u00e9culaire de l\u2019Universit\u00e9 d\u2019\u00c9tat du Colorado (CSU). Son laboratoire utilise une combinaison de microscopie de fluorescence avanc\u00e9e, de g\u00e9nie g\u00e9n\u00e9tique et de mod\u00e9lisation computationnelle pour \u00e9tudier la dynamique de la r\u00e9gulation des g\u00e8nes dans les cellules vivantes des mammif\u00e8res. Son laboratoire a aid\u00e9 \u00e0 lancer l\u2019imagerie de la dynamique de traduction en temps r\u00e9el de l\u2019ARNm unique dans les cellules vivantes1<\/sup>. Dr. Stasevich a obtenu son baccalaur\u00e9at en physique et en math\u00e9matiques de l\u2019Universit\u00e9 du Michigan, Dearborn, et son doctorat en physique de l\u2019Universit\u00e9 du Maryland, College Park. Il est pass\u00e9 \u00e0 la biophysique exp\u00e9rimentale en tant que chercheur postdoctoral dans le laboratoire du Dr. James G.McNally \u00e0 l\u2019Institut national du cancer. Pendant ce temps, il a d\u00e9velopp\u00e9 une technologie bas\u00e9e sur la microscopie de fluorescence pour aider \u00e0 \u00e9tablir des mesures de r\u00e9f\u00e9rence de la dynamique des prot\u00e9ines dans des cellules vivantes. Le Dr. Stasevich a ensuite d\u00e9m\u00e9nag\u00e9 \u00e0 l\u2019Universit\u00e9 d\u2019Osaka, o\u00f9 il a travaill\u00e9 avec le Dr. Hiroshi Kimura en tant que Membre de la Recherche postdoctorale \u00e9trang\u00e8re de la Japan Society for the Promotion of Science Foreign. L\u00e0-bas, il a aid\u00e9 \u00e0 cr\u00e9er la technologie pour l\u2019imagerie des prot\u00e9ines endog\u00e8nes et leurs modifications post-traduction in vivo<\/em>. Cela lui a permis d\u2019imaginer la dynamique des modifications \u00e9pig\u00e9n\u00e9tiques de l\u2019histone dans des cellules vivantes lors de l\u2019activation du g\u00e8ne pour la premi\u00e8re fois2<\/sup>. Avant de se joindre \u00e0 la facult\u00e9 \u00e0 la CSU, le Dr. Stasevich a pass\u00e9 un an en tant que chercheur invit\u00e9 au Campus de recherche Janelia HHMI, o\u00f9 il a appliqu\u00e9 la microscopie de fluorescence de super r\u00e9solution pour am\u00e9liorer la r\u00e9solution spatiotemporelle de l\u2019imagerie prot\u00e9ique endog\u00e8ne dans les cellules vivantes.<\/p>\n

1. Morisaki, T. et al. Real-time quantification of single RNA translation dynamics in living cells.
\nScience 352, 1425\u20131429 (2016).
\n2. Stasevich, T. J. et al. Regulation of RNA polymerase II activation by histone acetylation in single living cells. Nature 516, 272\u2013275 (2014).<\/p>\n

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